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High-throughput small molecule screenings in model plants are of great value to identify compounds that interfere with plant developmental processes. In academic research, the plant
Arabidopsis thaliana
is the most commonly used model organism for this purpose. However, compared to plant cellular systems,
A. thaliana
plants are less amenable to develop high-throughput screening assays. In this chapter, we describe a screening procedure that is compatible with liquid handling systems and increases the throughput of compound screenings in
A. thaliana
seedlings.