Front Oncol: World's first, saliva screening detects early cancer!

Saliva sieves out early pharynx cancer, or will be possibleMay 19 (UPI) -- Australian bioscientists have developed a salivare reagent that has successfully detected early throat cancer from a "healthy person without any symptoms,"May 19After a 15-minute surgical removal, the patient was curedrelated content in the form of "case reports" published in the journal Frontiers in Oncology" is the world's first non-invasive screening study of oral and pharynx cancer (OPC)The idea is to identify human papillomavirus (HPV)-16 DNA from subjects' saliva samplesIf it can be studied and validated in a wider range of healthy populations, we may be one step closer to promoting routine screening for oral cancerMedscape commentedSaliva tests lasting 36 months show edified positive for HPVpersistent HPV infection has become one of the main causes of oral cancerA review published in the New England Journal of Medicine in January 2020 said the number of cases of oral cancer caused by or associated with HPV caused by high-risk genotype HPV-16 is increasing year by yearIn the United States, the proportion diagnosed with HPV-positive pharynx cancer rose from 16.3 per cent in the 1980s to more than 72.7 per cent in the 2000s" our study found that HPV-16 DNA in patients with pharynx cancer may fall directly into saliva or act as a biomarker for early diagnosisCommunications author Chamindie Punyaa, associate professor at the Institute of Health and Biopharmaceutical Innovation at the Queensland University of Technology, Australia, saidis understood to have started oral HPV-16 DNA epidemiology research in May 2016-October 2017 by the saliva and body fluid biopsy conversion research team at the School of Biological Sciences, where Chamindie Punyadeera is based, in collaboration with a number of medical institutions to recruit 665 healthy people into the groupall subjects as required, leaving a sample mouthwashThe standard process for sampling and analysis is that the subjects use a specific concentration of oral salt solution, rinse for 1-2 minutes, and then leave samples in the test tubeThe team used self-developed reagents to analyze the samples for viral genotype and viral loadresults showed that three subjects were positive for persistent HPV-16 DNAAmong them, a 63-year-old white male, in the 6th, 12th and 36th months of the test, the HPV-16 virus load increasedFrom the initial 3.43 copy/50 ng to 1281.69 copy/50 ng at the 36th monththe man was invited to participate in the clinical assessment and discussion of the otolaryngology department at the invitation of the research team and under the supervision of the Research Ethics Committee" 15 years ago, he successfully quit smoking, but was 45 years oldHe drinks some wine every day, about two glassesIs heterosexual, with at least five sexual partnersOral sexual contact is the usual foreplayinitial oral and pharynx clinical examinations - including touch and white light - were found to be no significant abnormalitiesThe electronic nasal laryngism mirror showed that the two tonsils were slightly asymmetrical (left and right) and the left tongue tonsil souli had mild vascular changes, but there was no evidence of malignant lesionsIn addition, MRIs in the mouth and neck did not find hidden lesions of the tonsils or tongue roots, nor did the lymph nodes in the neck swell"The details of these discussions are recorded in the Case Reportobtained the man's informed consent, the team treated him for a double-sided tonsil excision The operation took only 15 minutes The patient was discharged on the same day postoperative biopsy showed that the left tonsill had squamous cell carcinoma with a diameter of 2 mm 2 weeks after surgery, no positive evidence of HPV-16 DNA was found in the saliva At 2 months after the operation, the neck ultrasound did not find swelling of the lymph nodes in the neck " the man is currently undergoing postoperative follow-up We believe that by removing this small operation from the tonsils, he would be able to obtain a cure and have a very low recurrence rate Chamindie Punyadda said Figure B for the postoperative left tonsill tissue slice found 2mm non-horny squamous cell carcinoma, the focal matrix immersion 1mm, cut edge is clear /Frontiers in Oncology hpv-induced pharynx cancer, lack of routine screening the research team said the previous lynx case is the world's first, through saliva screening detected, and then confirmed by histological pathology asymptomatic recessive larynx For clinical reasons, it has many implications first, it suggests that clinical, the link between saliva HPV-DNA rise and the course of oral cancer should be fully assessed has been studied in the past to prove that there is a certain degree of positive correlation between the two In 2016, the Journal of the American Medical Association, the Journal of Oncology, reported that a positive oral HPV-16 was found to be 22-fold higher for 96,650 subjects, four years after an average follow-up The conclusion suggests that simple oral mouthwash sample collection can provide forward-looking "biomarkers" for the risk of squamous cancer in the mouth and throat and the previous study further found that HPV viral loadcontinues continue to rise or indicate progression second, the definitive diagnosis of oral cancer is still mainly for invasive biopsy and genome sequencing techniques In particular, HPV-induced oral cancer has been lacking standard or routine screening procedures usually, patients may only be detected if the condition progresses to a later stage with obvious symptoms Patients can also get a better prognosis if saliva screening is possible, or if early screening and treatment of pharynx cancer can be significantly promoted again, it hints at the importance of promoting widespread hpv vaccination " In the 1980s, the majority of people with throat cancer were in their 70s, most of whom had a history of drinking or smoking Only a few cases are related to HPV infection But now throat cancer patients are often middle-aged men who are already husband or father, with better economic conditions, no smoking or alcoholism Men are three times more likely than women to be diagnosed with HPV-related throat cancer According to the New York Times, the main reasons for this situation include people starting sex earlier, having more sexual partners, and making more oral sexual contact studies have shown that the vast majority of HPV infections are wiped out by the body's immune system within 1-2 years And oral sexual contact infection HPV, if not timely removal, or have a 10-30 year incubation period Therefore, the HPV vaccine, or can prevent oral cancer, reduce the incidence To date, however, there is still a lack of data support for the preventive effect in 2013, actor Michael Douglas was diagnosed with throat cancer The Guardian quoted him as saying: "I don't want to go into more detail, this cancer is caused by HPV infection, and it is caused by oral contact." "/AP " As the world's first single case, this study is not direct evidence of support for saliva screening HPV in the general population But it proves that we can use a low-cost, non-invasive way to screen for oral cancer caused by HPV infection in high-risk groups, and to evaluate the effectiveness of treatment in this way Chamindie Punyadda said