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Front Neurol: Comprehensive bioinformatics analysis of potential mRNA and miRNA regulatory network in mice with ischemic stroke treated by electroacupuncture

 Last Update: 2021-10-20
 Source: Internet
 Author: User

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EA (EA) treatment of ischemic cerebral stroke complicated molecular mechanism is unclear
.

Recently, more evidence has revealed the important role of microRNA (miRNA)-mRNA network in ischemic stroke

Stroke

The researchers established a mouse model of middle cerebral artery occlusion (MCAO) and performed electroacupuncture on mice with ischemic stroke
.

Use behavior test and infarct area measurement to measure the effect of electroacupuncture treatment

Then, RNA sequencing was performed to analyze the differentially expressed genes (DEG) and functional enrichment between the EA and the control group
.

In addition, a protein-protein interaction (PPI) network was established, and the pivot genes were screened through Cytoscape

Then, RT-qPCR was used to verify the central genes and predicted miRNAs as key biomarkers
.

Finally, a miRNA-mRNA network was constructed to explore the potential mechanism of EA in ischemic stroke
.

Analysis shows that electroacupuncture can significantly reduce the neurological deficits of the affected limbs and reduce the infarct size of MCAO model mice
.

A total of 174 significant DEGs were identified between EA and the control group, including 53 up-regulated genes and 121 down-regulated genes

However, only four miRNAs were significantly up-regulated and showed a beneficial effect of EA treatment
.

Finally, a comprehensive analysis of the results determined that miR-425-5p-Cdk1, mmu-miR-1186b-Prc1, mmu-miR-434-3p-Prc1 and mmu-miR-453-Prc1 miRNA-mRNA networks are key networks regulated by EA And is related to ischemic stroke

miR-425-5p-Cdk1, mmu-miR-1186b-Prc1, mmu-miR-434-3p-Prc1, and mmu-miR-453-Prc1 miRNA-mRNA networks are key networks regulated by EA and related to ischemic stroke

The key DEG, miRNA and miRNA-mRNA regulatory networks have been identified.

Original: Wu C, Zhao L, Li X, Xu Y, Guo H, Huang Z, Wang Q, Liu H, Chen D, Zhu M.

Wu C, Zhao L, Li X, Xu Y, Guo H, Huang Z, Wang Q, Liu H, Chen D, Zhu M.
Integrated Bioinformatics Analysis of Potential mRNA and miRNA Regulatory Networks in Mice With Ischemic Stroke Treated by Electroacupuncture.
Front Neurol.
2021 Sep 10;12:719354.
doi: 10.
3389/fneur.
2021.
719354.
PMID: 34566862; PMCID: PMC8461332.
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