Front Immunol: Immunosuppressive therapy modulates to varying degrees the humoral and T cell specific responses of rheumatoid arthritis patients to the COVID-19 mRNA vaccine.

Objective: To evaluate the SARA-COV -2 specific immune response induced by anti-regional binding domain (RBD) antibodies and T cell specific immune responses in patients with rheumatoid arthritis (RA) treated with different immunosuppressive therapies, and Safety of the clinical impact of vaccines on disease activity
.

To evaluate the SARA-COV -2 specific immune response induced by anti-regional binding domain (RBD) antibodies and T cell specific immune responses in patients with rheumatoid arthritis (RA) treated with different immunosuppressive therapies, and the vaccine Safety of clinical effects of disease activity
.

Methods: The medical staff and patients with rheumatoid arthritis who completed the BNT162b2-mRNA vaccination in the past 2 weeks were selected as the research subjects
.

The serological response was assessed by quantifying anti-RBD antibodies, while the cell-mediated response was assessed by quantifying the interferon (IFN)-g-response to stimulating peptides by a whole blood test

Results: We prospectively recruited 167 HCW and 35 RA patients
.

Although CTLA-4 inhibitors (median: 465BAU/mL, IQR: 103~1189, p<0.

Figure 1.
Antibody and T cell responses of patients with rheumatoid arthritis after being vaccinated with SARS-CoV-2 vaccine
.

167 HCW and 35 RA patients were divided into 4 groups according to drug treatment: TNF inhibitor with or without DMARD group (n=7), IL-6 inhibitor with or without DMARD/CCS group (n=8), CTLA-4 inhibitor with or without DMARD/CCS group (n=13), DMARD with or without CCS group (n=7)

Figure 1.

Figure 2 Flow cytometry detection of IFN-gS specific T cell response
.

HCW (n=7) and RA (n=15) peripheral blood mononuclear cells (PBMC) were stimulated in a stimulating peptide pool for 24 hours in vitro, and the frequency of IFN-g-specific T cells was detected by flow cytometry

Figure 2 Flow cytometry detection of IFN-gS specific T cell response

This study showed for the first time that the antibody-specific and whole blood stimulatory T cell responses induced by the new coronary pneumonia gene vaccine appeared in most RA patients, who adopted the strategy of suspending immunosuppressive therapy during vaccination

Picchianti-Diamanti A, Aiello A,et al, ImmunosuppressiveTherapies Differently Modulate Humoral- and T-Cell-Specific Responses to COVID-19 mRNA Vaccine in Rheumatoid Arthritis Patients .

Differently Modulate Humoral- and T ImmunosuppressiveTherapies-the Cell-Specific Responses to COVID-19 mRNA Vaccine in Rheumatoid Arthritis Patients in this message