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From Proteomics to Systems Biology: MAPA, MASS WESTERN, PROMEX, and COVAIN as a User-Oriented Platform

 Last Update: 2020-11-07
 Source: Internet
 Author: User

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protein western blot

types mass spectrometry

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Genome sequencing and systems biology are revolutionizing life sciences. Proteomics emerged as a fundamental technique of this novel research area as it is the basis for gene function analysis and modeling of dynamic protein networks. Here a complete proteomics platform suited for functional genomics and systems biology is presented. The strategy includes MAPA (mass accuracy precursor alignment;) as a rapid exploratory analysis step; MASS WESTERN for targeted proteomics; COVAIN ( ) for multivariate statistical analysis, data integration, and data mining; and PROMEX ( ) as a database module for proteogenomics and proteotypic peptides for targeted analysis. Moreover, the presented platform can also be utilized to integrate metabolomics and transcriptomics data for the analysis of metabolite–protein–transcript correlations and time course analysis using COVAIN. Examples for the integration of MAPA and MASS WESTERN data, proteogenomic and metabolic modeling approaches for functional genomics, phosphoproteomics by integration of MOAC (metal-oxide affinity chromatography) with MAPA, and the integration of metabolomics, transcriptomics, proteomics, and physiological data using this platform are presented. All software and step-by-step tutorials for data processing and data mining can be downloaded from.

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