Epimedium extract

Epimedium cordifolia, a Berberidaceae plant, has high medicinal value Wei Xing GUI Jing: Xin, Gan, Wen Main functions of liver and kidney meridian: tonifying kidney yang, strengthening muscles and bones, eliminating wind dampness [source] the dried leaves of Epimedium brevicornum Maxim., Epimedium sagittatum Maxim., Epimedium pubescens Maxim., or Epimedium koreanum Nakai In summer and autumn, the stems and leaves are cut when they are luxuriant The stems, thick stems and impurities are removed and dried in the sun or in the shade Epimedium perennial herbs, 30-40 cm high The rhizome is long, transverse, hard, with many fibrous roots The leaves are 2-fold, 3-fold, 9 lobules, with long stalk, thin leathery lobule, ovate to long oval, 4.5-9cm long, 3.5-7.5cm wide, apex sharp, margin serrulate, serrate apex spiny, base deep heart-shaped, lateral lobule base oblique, upper part sparsely hairy when young, hair falling off gradually after flowering, and lower part villous There are 4-6 flowers in racemes, the axis of the inflorescence is glabrous or occasionally hairy, the pedicel is about 1cm long; there are bracts at the base, ovate lanceolate, membranous; the flowers are large, about 2cm in diameter, yellow white or milky white; there are 8 sepals, ovate lanceolate, 2 rounds, 4 small ones outside, different shapes, 4 large ones inside, homomorphic; petals 4, nearly round, with long distance; stamens 4; pistils 1, style long Follicles fusiform, 2-lobed at maturity The flowering period is from April to May The fruit period is from May to June It grows in shady woods and thickets It is distributed in Heilongjiang, Jilin, Liaoning, Shandong, Jiangsu, Jiangxi, Hunan, Guangxi, Sichuan, Guizhou, Shaanxi and Gansu 2 Epimedium sagittatum is a perennial herb with a height of 30-50 cm The rhizome was tuberculate Root leaves 1-3, 3 compound leaves, leaflets ovate to ovate lanceolate, 4-9cm long, 2.5-5cm wide, apex sharp or acuminate, with fine bristles on the edge, heart-shaped at the base, asymmetric at the base of lateral leaflets, oblique and large lateral lobes, triangular, small and nearly round inner lobes; cauline leaves are often opposite to the top, similar to root leaves, with oblique arrow shaped heart-shaped at the base , lateral lobes extra large and apex acuminate The flowers are mostly clustered into racemose or lower branches to form a panicle, the flowers are small, the diameter is only 6-8 mm, and the petals are short or close to no distance The flowering period is 2-3 months The fruit period is from April to May Born under the bamboo forest on the hillside or in the rock crevices beside the road It is distributed in Zhejiang, Anhui, Jiangxi, Hubei, Sichuan, Taiwan, Fujian, Guangdong, Guangxi, etc 3 The leaf surface and petiole of Epimedium pubescens are densely fluffy It is mainly produced in Sichuan, with good and stable quality 4 The small leaves of Epimedium koraiensis are large, 4-10cm long, 3.5-7cm wide, and the apex is long and pointed The leaves are thin Mainly produced in the northeast Medicinal and food use [processing] Epimedium: pick up impurities, remove stem, cut into silk and sift out debris Burn Epimedium: heat and melt the lanolin sleeve in the pot, remove the residue, add epimedium and stir fry until the lanolin oil is almost exhausted, take it out and cool it (100jin of epimedium, 25jin of refined lanolin oil) pungent, sweet and warm Return to liver and kidney channel [function indications] Tonifying Kidney Yang, strengthening muscles and bones, removing wind and dampness It is used for impotence and spermatorrhea, weakness of muscles and bones, rheumatism and arthralgia, numbness and contracture, climacteric hypertension [application] 1 It is used for impotence of kidney deficiency, premature ejaculation of spermatorrhea, impotence of waist and knee, cold and chilly of limbs It can be used to treat impotence, such as Festuca, Cornus, Cistanche, etc.; it can be used to treat impotence, such as eucommia, Morinda, dog's back, etc 2 It is used for cold dampness arthralgia or limb contracture numbness It can be used in combination with Wei Lingxian, Morinda, cinnamon, angelica, chuanxiong, etc [usage and dosage] decoction, 3-9g Suitable for people 1 Suitable for impotence, uterine cold infertility, Yang deficiency hypertension, menopause 2 Those who are weak in Yin and strong in Yang are forbidden to take it Food therapy has androgen like effect; it can improve the immune function of the body; it can expand the peripheral blood vessels, improve microcirculation, increase blood flow, and increase coronary flow; it has antitussive, expectorant, anti hypoxia, sedative and anti convulsive effects; it has inhibitory effect on poliovirus and a variety of enteroviruses [purpose] for kidney yang deficiency, impotence, frequent urination, infertility; rheumatism arthralgia, numbness and contracture of limbs, weakness of muscles and bones, hard walking; kidney yang deficiency, shortness of breath and cough Medical effect [chemical composition] Epimedium contains icariin, icarisoside and so on Epimedium Korean contains icariin, epimedioside a, B, C, D, e and so on Epimedium sagittatum contains icariin, dehydrated icariin-3-o-a-rhamnoside and icariin-3-o-a-rhamnoside, Quercetin, et al Research progress of chemical components: a little [physical and chemical identification] 1 Epimedium powder 0.5g, 10 ml ethanol, warm soak for several minutes, filtration, hydrochloric acid magnesium reaction of filtrate, red 2 Thin layer chromatography (1) Epimedium (2) Epimedium (3) Epimedium (4) Epimedium glycoside thin layer chromatography sample solution: take powder 0.5g, 10 ml ethanol, warm soak for 30 minutes, filtration, evaporation of filtrate, 1ml solution was added to ethanol as a sample Control solution: icariin and ethanol were mixed into each 1ml containing 0.5mg as control solution Expand: silica gel H-cmC (0.5%) thin layer board, with butyl acetate: formic acid: water (52: 40: 40), expand the distance 15cm color: spray 1% three aluminum chloride ethanol solution, under ultraviolet lamp (365nm) inspection The color is orange red; the test sample shows the same spot as the corresponding position of the photograph Research progress of component analysis: slightly [content determination] 1 Determination of total flavonoids: precisely measure 0.5ml of test solution under the determination of icariin, place it in a 50ml measuring flask, add methanol to the scale, shake well, as the test solution Take the icariin reference, add methanol to make a solution containing 10 μ g per 1ml, As the reference solution, take the test solution and the reference solution respectively, take the reagent as the blank, measure the absorbance at the wavelength of 270nm, and calculate it The leaves of this product are calculated as dry products, and the total flavonoids are calculated as icariin (c33h40o15), No less than 5.0% 2 Determination of icariin: chromatographic conditions and system applicability test: use octadecylsilane bonded silica gel as filler; acetonitrile water (30:70) as mobile phase; detection wavelength is 270nm Theoretical plate number shall not be less than 1500 according to icariin peak calculation Preparation of control solution: weigh accurately appropriate amount of icariin control, add methanol to make a solution containing 0.1mg/1ml, That is to say, preparation of test solution: take about 0.2g of this product's leaf powder (passing 40 mesh sieve) (at the same time, take another sample of this product's leaf powder to determine water content), weigh accurately, put it into a 50ml conical flask with a plug, add 20ml of dilute ethanol precisely, close the plug, weigh it, ultrasonic treatment for 1h, weigh it again, use dilute ethanol to make up the weight lost, shake it well, filter it, take the continuous filtrate, Determination method: take 10 μ l of the control solution and the test solution respectively and inject them into the human liquid chromatograph for determination The leaves of this product are calculated according to the dried product and contain icariin (c33h40o15), which shall not be less than 5.0% Research progress of component analysis: slightly [pharmacological function] 1 Androgen like effect: the fluid extract has the effect of promoting the semen secretion of dogs, The weight of prostate gland, seminal vesicle and levator ani muscle were increased after administration, It is proved that Epimedium has androgen like effect 2 The effect on cardiovascular system: the extract and decoction of Epimedium have the functions of increasing coronary flow, resisting hypoxia, protecting myocardial ischemia and reducing blood pressure in mice, rats, guinea pigs, isolated and in situ rabbit hearts and anesthetized dogs, respectively Flavonoids are the main active components 3 Effect on immune function: Epimedium 70% methanol extract 200mg / kg or 500mg / kg can enhance the phagocytic ability of reticuloendothelial system, It can accelerate the clearance rate of carbon particles in mice 4 Promote the growth of bone: Epimedium injection can significantly promote the growth of chicken embryo bone and the synthesis of chicken embryo femur proteoglycan in vitro Epimedium extract can inhibit the bone absorption of rats after testis removal, and its polysaccharide can make mouse bone marrow cells proliferation In addition, it has anti-aging, anti fatigue, anti-virus and other effects