Effects of Perilla Seed Extract on survival, growth and biochemical composition of allogynogenetic crucian carp

Cheng Yongxu, Key Laboratory of aquatic germplasm resources and aquaculture ecology, Ministry of agriculture, Shanghai University of fisheries, studied the effect of different content of perilla seed extract (PSE) in feed on the growth, survival and fish composition of allogynogenetic crucian carp, providing theoretical and practical basis for the development and utilization of Perilla Seed Extract in aquatic feed Materials and methods a total of 300 healthy experimental fishes of certain specifications were selected, which were domesticated in 15 glass tanks for 2 weeks, and the temperature was controlled at 25 ℃ ~ 28 ℃ Twenty four hours before the start of the experiment, 15 fish were randomly placed in each aquarium Each treatment group was provided with 3 parallel tanks, and 15 aquariums were totally placed in 5 treatments Five groups (6 fish / group) were randomly selected from the remaining fish to weigh, measure the body length, weigh the viscera after dissection, and store them in - 65 ℃ freezer as the initial condition of the experiment After 8 weeks, the amount of perilla seed extract was 0 (group A), 0.01% (group B), 0.03% (Group C), 0.05% (Group D) and 0.08% (Group E), respectively During the experiment, the fish were fed twice a day at a daily dosage of 3% ~ 5% of the body weight In order to accurately calculate the feeding amount of fish, the residual bait should be sucked and dried in time every day During the experiment, weigh it every two weeks At the end of the experiment, the body weight and length of all the fish were measured, then all the fish were dissected, the viscera were taken out, and the weight of viscera was weighed (accuracy 0.01g) All the samples were stored in - 65 ℃ freezer for biochemical determination Results the feeding, growth and feed utilization efficiency of allogynogenetic crucian carp can be seen from the table below At the end of the experiment, the survival rate of allogynogenetic crucian carp with Perilla seed extract was 100%, while that of the control group was 95.6% The body weight gain of group B and group C was significantly higher than that of the other three groups, especially that of group C was 99.50% The visceral body ratio of the five treatment groups was also significantly lower than that of the other groups in group C (12.42%) In terms of specific growth rate and weight gain rate, the specific growth rate and body weight gain rate of C group were higher than that of the control group The feed efficiency of group C (42.74%) was significantly higher than that of the control group (32.24%) Similarly, the protein efficiency of group C was significantly higher than that of the control group Therefore, based on the above experimental indicators, it can be clearly seen that group C has a significant growth promoting effect on Allogynogenetic crucian carp when adding 300 mg / kg perilla seed extract to the basic feed In group C, the weight gain rate was the highest (99.5%), feed efficiency and protein efficiency were the highest (42.74% and 118.72%, respectively), and the ratio of body to body was the lowest Compared with the control group, the content of water, crude protein and crude fat in the body of allogynogenetic crucian carp treated with PSE was not significantly different; the content of water was about 76%, the content of protein was about 16% ~ 17% of body wet weight (about 70% of body dry weight), and the content of fat was about 4% ~ 5% of body wet weight, among which the content of fat in group C was the lowest (4.11%), which was lower than that in the control group The highest protein content was 17.03%, which was higher than that of the control group Compared with the control group, the content of water and crude protein in the viscera of allogynogenetic crucian carp had no significant difference, the content of water was between 67% and 70%, and the content of crude protein was between 8% and 10% of visceral wet weight, but the content of crude fat was significantly different, among which the content of visceral fat in group C was the lowest, 5.23% of visceral wet weight, followed by Group D In this experiment, compared with the control group, the fat content of viscera can be significantly reduced by adding PSE of 300mg-500mg / kg Composition of body and visceral fatty acids in Allogynogenetic crucian carp The results showed that there was no significant difference in the content of SFA in all groups of experimental fish in terms of body fatty acid composition; the content of MUFA in group A was up to 60.17%, while that in group C was down to 53.16% There was no significant difference in the content of PUFA and HUFA in all groups, especially HUFA with the addition of EPS The increase of dosage has a significant increasing trend The highest was 13.37% in Group E, 12.42% in group C, 11.84% in group A, N3 / N6, and the highest in group C As far as the fatty acid composition of viscera is concerned, there is no significant difference in the percentage of SFA of experimental fish in each group; there is no significant difference in the content of MUFA in each group; the highest percentage of PUFA in each group is 16.77% in group A, 16.74% in group C, 12.14% in Group E, and there is significant difference between groups a, C and E In general, the content of PUFA and HUFA in viscera of group C was higher than that of other PSE groups, while the ratio of N3 / N6 was lower than that of body value Summary 1 The experiment group C added 300 mg / kg perilla seed extract to the basic diet had significant growth promoting effect on the Allogynogenetic crucian carp 2 Adding 300 mg / kg PSE to the feed can promote the utilization of exogenous absorbed fat, enhance the consumption of absorbed fat, provide the energy demand of energy metabolism and growth of the body, so as to save protein and promote the transformation of absorbed protein The results showed that the body weight increased significantly, the body protein content was higher, and the body to body ratio of group C was smaller, which fully showed that the significant body weight gain of fish was mainly body weight gain, and mainly protein increase 3 Adding a certain amount of PSE can promote the transformation of PUFA and HUFA from viscera to body.