Effect of Ginkgo biloba extract on pulmonary neutrophil function in rats with severe acute pancreatitis

Recently, researchers from the general surgery department of the Second Affiliated Hospital of Wenzhou Medical College published a paper to explore the effect of Ginkgo biloba extract (GBE) on the function of alveolar neutrophils (PMN) in rats with severe acute pancreatitis (SAP) and lung injury The results showed that the expression of CD11b / CD18 and ICAM-1 on the surface of PMN was up-regulated in SAP with lung injury, which resulted in PMN adhesion to pulmonary vascular endothelial cells, and then activated PMN to release ne, aggravating lung injury GBE can decrease the expression of ICAM-1 and CD11b / CD18, and prevent PMN from adhering and activating in the lung, so as to reduce lung injury This article was published in the 4th issue of Chinese Journal of integrated traditional and Western medicine in 2014 48 adult SD rats were randomly divided into three groups: sham operation group, SAP group and GBE treatment group, 16 rats in each group The sap model was successfully made by retrograde injection of 5% sodium taurocholate into the pancreaticobiliary tract The sham operation group only underwent duodenal overturning The GBE treatment group received GBE intervention on the basis of SAP model The sham operation group and SAP group were given the same amount of normal saline The rats were killed at 6h and 12h after operation, respectively Lung tissue samples were collected for lung tissue injury score, and the wet / dry weight ratio (w / D) and myeloperoxidase (MPO) activity of lung tissue were measured PMN was separated and collected by bronchoalveolar lavage The content of neutrophil elastase (NE) in bronchoalveolar lavage fluid (BALF) was detected by ELISA, and the percentage of CD11b / CD18 double positive cells on the surface of PMN was detected by flow cytometry Western blot was used to detect the expression of ICAM-1 and ne Compared with the sham operation group, the lung tissue of SAP group showed obvious histopathological damage, the lung tissue injury score, the content of NE in w / D, MPO and BALF increased, the expression of ICAM-1 and NE protein in the lung tissue increased significantly, the percentage of double positive cells of CD11b / CD18 on the surface of PMN increased significantly (P < 0.01); compared with SAP group, the lung histopathological damage of GBE group was significantly reduced All the indexes were significantly decreased (P < 0.01).