Effect of extract from coal tar pitch smoke on the expression of COX-2 mRNA in BEAS-2B cells

Recently, researchers from the Department of labor hygiene, School of public health, Zhengzhou University published a paper to explore the effect of coal tar pitch smoke extract (CTPE) on the expression of cyclooxygenase-2 (COX-2) mRNA in human bronchial epithelial cells It is suggested that CTPE can induce COX-2 mRNA expression in human bronchial epithelial cells at the transcriptional and posttranscriptional levels This article was published in the 4th issue of Journal of Zhengzhou University (Medical Edition) in 2014 RT-PCR was used to determine the effect of CTPE at different concentrations (2,4,8 mg / L) on the expression of COX-2 mRNA in BEAS-2B cells The effect of actinomycin D (1 mg / L) on COX-2 gene transcription in BEAS-2B cells was observed after 30 minutes of CTPE treatment The COX-2 mRNA expression of BEAS-2B cells was stimulated by ZnSO4 solution After ZnSO4 was removed, 1 mg / L act D was added for 30 min to detect the change of COX-2 mRNA expression at different time points (0, 2, 4 h) of CTPE 2,4,8 mg / L CTPE could increase COX-2 mRNA expression level in BEAS-2B cells (F group = 71.750, f time = 93.120, f interaction = 17.300, P < 0.001), 2 mg / l was the best dose Pretreatment of cells with act D (1 mg / L) for 30 min reduced the induction of COX-2 mRNA expression by CTPE (fact d = 174.203, fctpe = 260.312, f interaction = 188.521, P < 0.001) After ZnSO4 stimulation and act D termination of COX-2 mRNA synthesis, there was statistically significant difference in COX-2 mRNA expression between CTPE group and control group at different time points (F group = 365.700, f time = 37.780, f interaction = 6.240, P < 0.001).