Effect of cigarette extract on alveolar epithelial cells and intervention of reduced glutathione

Recently, researchers from the respiratory department of the second hospital of Hebei Medical University published a paper, aiming to use different concentrations of cigarette extract (CSE) to stimulate human A549 cells cultured in vitro, and observe the morphological changes of cell growth and proliferation inhibition By detecting the activity of superoxide dismutase (SOD) and the level of malondialdehyde (MDA) in the supernatant, the effects of smoking on human lung epithelial cells and the reversion of GSH on the injury were studied The results show that 1cse can inhibit the growth of alveolar epithelial cells in a dose and time-dependent manner, and 2cse can cause oxidative damage to alveolar epithelial cells 3GSH can reverse the oxidative damage of alveolar epithelial cells induced by smoking This article was published in the 2007 issue of clinical highlights in 2015 Firstly, A549 cells were cultured in 0%, 2.5%, 5.0%, 7.5%, 10.0% and 12.5% CSE medium The cell viability was measured by 3 - (4,5-dimethylthiazol-2) - 2,5-diphenyltetrazolium bromide (MTT) method, and the cell proliferation morphology was observed by phase contrast microscope According to the results, the experiment was divided into three groups: blank control group, 2.5% CSE group, 5.0% CSE group, 7.5% CSE group, 7.5% CSE + GSH group (GSH 1mmol / L) The SOD activity and MDA level of cell supernatant were detected by colorimetry The growth density of A549 cells decreased, floating cells increased and adherent cells atrophied after the treatment of 1cse CSE had a strong inhibitory effect on A549 cell proliferation 2cse can promote the secretion of MDA and decrease the activity of SOD in A549 cells in a dose-dependent manner The MDA level of 37.5% CSE + GSH group was significantly lower than that of 7.5% CSE group, and the SOD activity was higher than that of 7.5% CSE group.