Echinacea polyphenols

【】Caffeic acidCh10rogenic acidEchinacoside VerbascosideCynarin :30% 【】,、、、。"Identification" take this product with methanol to make 0.5% solution for test ingeTake the standard chrysanthemum acid, coffee acid, chlorogenic acid, echinaceag plus methanol to make each containing 1 mg / ml solution for controlTake 1.0g of standard control of echinacea with methanol 30 ml, ultrasound extraction for 30 minutes, the filter concentrates to 1 ml for controlTake the above three liquid each 6 sl, respectively, point on the same silicone G board, a point two board(1) is expanded in methanol-water-ice acetic acid (3:2:0.1) and UV lamp 365nm is viewed(2) is expanded at the upper level of acetate-forthic acid-water (7:2.5:2.5) and uv lamp 365nmthe above two plates, the corresponding position of the standard speckle appears the same color fluorescent spots, compared to the standard medicinal spots, the corresponding rate of 60%Dry weightlessness: No more than 4.0% (Appendix IX.G)burning residue: No more than 0.3% (Appendix IX.J)Heavy Metals: No more than 10ppm (Appendix IX.E)"Content Measurement" ashigh-efficient liquid chromatography (Appendix VI.D) measurementchromatography condition C18 columnflow phase A-ethyl;flow rate: 0.8ml/mindetection wavelength: 330nmThe number oftheory boards is 2000gradient "text-align: center;" src"/uploads/uploads/allimg/161221SH2a2a.jp0.jpg" style: "width: 510px; height: 143px;control fluid: take chrysanthemum acid standard with 70% methanol to make 0.1mg/ml solution backuptest fluid: take this product plus 70% methanol to make 0.2mg/ml solution backupthe determination method: take the above-mentioned two-liquid 5 sl injection liquid chromatography, and calculate the total polyphenol contentfunction to suppress bacteria, anti-infectionstorage is cool, dry and light-proof