echemi logo
Product
  • Product
  • Supplier
  • Inquiry
    Home > Biochemistry News > Biotechnology News > Determination of melting temperature (Tm) of salmon sperm DNA by UV method

    Determination of melting temperature (Tm) of salmon sperm DNA by UV method

    • Last Update: 2022-08-30
    • Source: Internet
    • Author: User
    Search more information of high quality chemicals, good prices and reliable suppliers, visit www.echemi.com
    The sound of cicadas is coming to summer, the scorching sun is shining, and the heat is hot.


    No surprise, frozen watermelon, ice cola, ice cream assassin, air conditioner.


    To sum up, it can be seen that organisms are very sensitive to changes in temperature, and changes in temperature affect everything in the world


    Different types of DNA have different Tm values: the higher the GC content, the higher the Tm (due to the 3 hydrogen bonds between guanine-cytosine (G≡C) nucleotides, and the adenine-thymine (A=T) There are two hydrogen bonds between ), and the energy required for G≡C nucleotide dissociation is greater than the energy required for A=T base pair


    Figure: Melting curve of salmon sperm DNA at 260nm (the case source is METTLER PUBLIC ACCOUNT)


     
    Our company has prepared application tips and complete sets of equipment for the majority of Ultraman experiments that believe in light, which can easily handle UV experiments that require temperature control of samples


    METTLER TOLEDO UV-Vis spectrophotometer + LAUDA water bath temperature control system combination scheme:

    This article is an English version of an article which is originally in the Chinese language on echemi.com and is provided for information purposes only. This website makes no representation or warranty of any kind, either expressed or implied, as to the accuracy, completeness ownership or reliability of the article or any translations thereof. If you have any concerns or complaints relating to the article, please send an email, providing a detailed description of the concern or complaint, to service@echemi.com. A staff member will contact you within 5 working days. Once verified, infringing content will be removed immediately.

    Contact Us

    The source of this page with content of products and services is from Internet, which doesn't represent ECHEMI's opinion. If you have any queries, please write to service@echemi.com. It will be replied within 5 days.

    Moreover, if you find any instances of plagiarism from the page, please send email to service@echemi.com with relevant evidence.