Cotton team reveals new mechanism of cotton callus fate regulation

Nanhu News (Correspondent Sun Weinan) Recently, the research results of the cotton genetic improvement innovation team of our school were published in The Plant Cell with the title of "GhTCE1-GhTCEE1 dimers regulate transcriptional reprogramming during wound-induced callus formation in cotton"

When a plant is mechanically injured, the plastic development process of the injured site is important for its wound repair and environmental adaptation

Fig.

Early in the laboratory, RNA-Seq analysis during embryogenesis (SE) in cotton cells identified a transcription factor GhTCE1 that was strongly induced during the early SE stage

Given that a large number of GhLTPs are enriched in the candidate targets, this study selected GhLTP2/3 which are highly expressed in the SE process, and confirmed that GhTCE1 can bind to the GhLTP2/3 promoter through yeast single hybrid, LUC, EMSA and ChIP-qPCR experiments.

Figure 2 GhTCE1 binds to the promoter of the target gene GhLTP2/GhLTP3 to activate its expression

In addition, this study also confirmed by genetic experiments that GhLTP2 and GhLTP3 have similar functions to GhTCE1

By screening the early callus cDNA yeast library, 10 candidate GhTCE1 interacting proteins were identified

Fig.

The results of this study found that the GhTCE1-GhTCEE1 interaction module acts as a molecular switch to coordinately regulate GhLTPs and ROS homeostasis to control the fate transition between disorganized callus proliferation and organized organ development.

The cotton genetic improvement innovation team of the National Key Laboratory of Crop Genetic Improvement of Huazhong Agricultural University has graduated Ph.

Reviewer: Yang Xiyan

【English summary】

Wounded plant cells can form callus to seal the wound site.

Paper link: https://doi.