Complete genome sequence map of fluffy tobacco and forest tobacco

The complete genome sequence map of fluffy tobacco and forest tobacco has been completed by Chinese tobacco This is the first complete genome sequence map of tobacco in the world, and the third complete genome sequence map of Solanaceae after potato and tomato genome The whole genome sequence map of fluffy tobacco and forest tobacco is the two maps with the largest genome, the highest assembly precision and the best assembly result Fluffy tobacco and forest tobacco are two ancestor species of cultivated tobacco The completion of the complete genome sequence map of fluffy tobacco and forest tobacco has important scientific value and strategic significance, will usher in a new era of tobacco scientific research and technological development, is an important milestone in the development of tobacco science, and marks that tobacco research has entered the era of genome since then The complete genome sequence map of fluffy tobacco and forest tobacco is completed by the most authoritative and powerful genome sequencing organization in the world According to the characteristics of high repetitive sequence content and large genome of tobacco, the bacterial artificial chromosome (BAC) was constructed by the whole genome shotgun method (WGS) combined with the second generation of high-throughput sequencing technology of Illumina To BAC) strategy for sequencing and assembly Using the strategy of whole genome shotgun (WGS) to construct inserted fragment libraries of different lengths for double terminal sequencing and assembly, and selecting BAC clones with a certain genome coverage, sequencing and assembling BAC clones, and then combining the two data for deep assembly, finally obtaining the high-quality tobacco genome sequence map, realizing the high-depth repeat sequencing of single base, and ensuring To prove the high accuracy of single base Through data analysis, the genome size of both fluffy tobacco and forest tobacco is 2.4GB, the coverage of euchromatic region is over 95%, the coverage of gene region is over 98%, and the whole genome sequence map (the first version) of contig N50 is over 20KB, scaffold N50 is more than 350 KB, which has reached the internationally recognized standard of high-quality fine map It is the two maps with the largest genome, the highest assembly precision and the best assembly result in the known plant genome sequence map, ranking the leading position in the world According to the current work, it is expected that the sequence map (the second version) will be available before the end of the year It is expected that the scaffold N50 will reach more than 1m, which is significantly higher than the first version of the map The whole genome sequence map of fluffy tobacco and forest tobacco is a part of China tobacco genome project As a major special project of tobacco industry, China tobacco genome project is a major scientific research project carried out by the whole industry of China tobacco It was launched on December 9, 2010, and has a full anniversary so far China tobacco genome project is mainly undertaken by national tobacco gene research center, Qingzhou Tobacco Research Institute of China Tobacco Corporation, Yunnan Tobacco Agricultural Science Research Institute, Guizhou Tobacco Research Institute, Heilongjiang Tobacco Science Research Institute and other units It brings together domestic and foreign power experts to closely track the current frontier of genomics, bioinformatics, genetics and other disciplines In line with the attitude of being responsible for science, history and industry, the exhibition has carried out overall planning and systematic implementation in tobacco structural genomics, functional genomics and molecular breeding to ensure that it is the world's leading level from technology to system At present, major projects have made breakthroughs and achievements in many fields On the basis of the complete genome sequence map of forest tobacco and fluffy tobacco, the genetic map and physical map drawing of fluffy tobacco and forest tobacco, and the genome sequencing of a large number of tetraploid cultivated tobacco will be carried out orderly; the creation of tobacco mutant library has reached 270000 and is undergoing large-scale identification and analysis; the construction of tobacco molecular genetic map and the location of important Mutation Genes According to the first version of the genome map and the joint analysis of the deep transcriptome sequencing of multiple samples, the analysis of the anabolic pathway of the main metabolites of tobacco is more in-depth, which is of great significance to the reduction of tobacco damage In general, the progress and achievements of major projects of tobacco genome project are at the forefront of tobacco science and technology in the world.