Clin Exp Immunol: Interferon-Gamma- and perforin-positive T cells in acquired aplastic anemia predict response to immunosuppressive therapy

Acquired aplastic anemia (aAA) is an autoimmune disease characterized by infiltration of T lymphocytes in the bone marrow and destruction of hematopoietic stem cells by effector cells

Acquired aplastic anemia (aAA) is an autoimmune disease characterized by infiltration of T lymphocytes in the bone marrow and destruction of hematopoietic stem cells by effector cells

Table 1: Characteristics of patients with developmental anemia enrolled in this study

Table 1: Characteristics of patients with developmental anemia enrolled in this study

The researchers recruited 55 newly diagnosed and untreated acquired aplastic anemia patients (40 males, 15 females, median age 21 years, range 5-60 years)

The researchers recruited 55 newly diagnosed and untreated acquired aplastic anemia patients (40 males, 15 females, median age 21 years, range 5-60 years)

Table 2: Median percentage and absolute number of T cells (per liter) in patients before and after initiation of immunosuppressive therapy (IST)

Table 2: Median percentage and absolute number of T cells (per liter) in patients before and after initiation of immunosuppressive therapy (IST)

Figure 1: Representative flow cytometry plots showing results in healthy controls, untreated non-severe aplastic anemia (NSAA), severe aplastic anemia (SAA), and very severe aplastic anemia ( Median percentage and absolute numbers of IFN-γ and perforin-producing CD5 T cells in patients with VSAA)

Figure 1: Representative flow cytometry plots showing results in healthy controls, untreated non-severe aplastic anemia (NSAA), severe aplastic anemia (SAA), and very severe aplastic anemia ( Median percentage and absolute numbers of IFN-γ and perforin-producing CD5 T cells in patients with VSAA)

Figure 2: Median percentage and absolute numbers of IFN-γ and perforin-positive CD5 T cells in healthy controls (children and adults), untreated patients (children and adults)

Figure 2: Median percentage and absolute numbers of IFN-γ and perforin-positive CD5 T cells in healthy controls (children and adults), untreated patients (children and adults)

Results showed higher median percentages and absolute numbers of interferon-gamma (IFN-γ) and perforin-positive CD5 T cells in untreated patients compared to healthy controls

Table 3: Correlation analysis of T cells before and after immunosuppressive therapy (IST) in patients

Table 3: Correlation analysis of T cells before and after immunosuppressive therapy (IST) in patients

Figure 3: Representative flow cytometry plots showing the neutralization of IFN-γ and perforin-positive CD5 T cells before and after initiation of immunosuppressive therapy (NR), partial (PR), and complete responder (CR) patients in non-responders Bit percentage and absolute number

Figure 3: Representative flow cytometry plots showing the neutralization of IFN-γ and perforin-positive CD5 T cells before and after initiation of immunosuppressive therapy (NR), partial (PR), and complete responder (CR) patients in non-responders Bit percentage and absolute number

Figure 4: Hemoglobin and platelet counts in partial and complete responders before and after immunosuppressive therapy (IST)

Figure 4: Hemoglobin and platelet counts in partial and complete responders before and after immunosuppressive therapy (IST)

Collectively, IFN-γ and perforin are essential effector cytokines for understanding the immunopathophysiology of acquired aplastic anemia

Collectively, IFN-γ and perforin are essential effector cytokines for understanding the immunopathophysiology of acquired aplastic anemia

Original source:

Original source:

Sharma V, Kumar P, Kumar R, Chakraborty S, Namdeo M, Sazawal S, Kanga U, Seth T, Mitra DK.

Sharma V, Kumar P, Kumar R, Chakraborty S, Namdeo M, Sazawal S, Kanga U, Seth T, Mitra DK.

 

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