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Chrysanthemum (Dendranthema grandiflora)

 Last Update: 2020-11-20
 Source: Internet
 Author: User

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This chapter provides reproducible methods for the transformation of
Dendranthema
�
grandiflora
‘Shuhou-no-chikara’ (standard) and ‘Lineker’ (spray) stem internode thin cell layers (TCLs) and conventional stem internode explants using
Agrobacterium
strains carrying a binary vector with β-glucoronidase (GUS) reporter and
npt
II selector genes. Transformation efficiencies are reported at the kanamycin selection (callus formation and plantlet rooting), GUS assay, polymerase chain reaction (
PCR
), and Southern analysis levels. Notes on regeneration improvement through the use of TCLs, as well as the effective use of sonication for both regeneration and generation of transformants, stimulation of
Agro
-infection and elimination of
Agrobacterium
, are included.

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