Chromosome-level genome assembly of human fungal pathogens reveals collinearity between geographically different species

Histoplasma capsulata

It is a type 2 fungal pathogen and the most common cause of fungal respiratory tract infections in immunocompetent hosts

Tissue cytoplasmThe species is a type 2 fungus, causing significant morbidity and mortality worldwide

And closely related pathogensBlastomyces,Paracoccidiosis,andCoccidiosisIt is a heat type 2 fungus that causes fungal infections in hosts with strong and weak immune functions (1) Histoplasmosis, a form ofTissue cytoplasm, Is an important cause of death in individuals with immunocompromised function (2).

It is popular in the Ohio and Mississippi river basins in the United States, and is also distributed all over the world, mainly in North America, South America and Africa

It exists in the soil in the form of saprophytic hyphae and produces asexual spores called conidia

Specifically, the Oxford nanopore technology and other long-reading sequencing tools have shown great prospects in the field of fungal genomics (13–16) In this study, we tested fiveTissue cytoplasmG217B, H88, G184AR, G186AR and WU24 belong to 4 different populations (5).
Combining long-read (Oxford Nanopore [ONT]) and short-read (Illumina) sequencing, weStart from scratchPut all the chromosomes together
.
The comparison of combinatorial genomes revealed the basic collinearity regions of chromosomes
.
Further examination of these genomes foundRYP2 type, A regulator of yeast stage growth, inTissue cytoplasmG186AR strain
.
Re-analysis of the high-throughput data set also showed that in the G217B and H88 strains, transposon genes and genes embedded in the rich regions of transposon showed more abundant transcription in the yeast stage
.
This study emphasizes that the complete genome assembly allows new insights to be obtained from the genome data set and will provide opportunities for future research of the organism
.