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6.
Product standards (biochemical reagents)
7.
Quality inspection
Like many other sterols, cholic acid can produce color reaction with furfural-concentrated sulfuric acid, but this method is not specific.
The concentration of furfural and sulfuric acid must be controlled to avoid interference by impurities
.
At present, this method is commonly used to determine the content of cholic acid in bile or finished products
(1) Preparation of cholic acid standard solution: accurately weigh 12.
5mg of cholic acid national standard, add 60% acetic acid solution to dissolve, and dilute to 25mL
.
(2) Drawing the standard curve: accurately measure 0.
4 mL, 0.
6 mL, 0.
8 mL, and 1.
0 mL of the bile acid standard solution, and place them in a test tube with a stopper, and fill each tube to 10 mL with a 60% acetic acid solution
.
Add 1 mL of freshly prepared 1% furfural solution to each tube, place in an ice bath for 5 minutes, add 13 mL of sulfuric acid solution (H 2 SO 4 : H 2 O=50:65, volume ratio), and keep in a water bath at 70°C for 10 minutes Take it out and place it in an ice bath immediately for 2 minutes, and measure the absorbance at a wavelength of 620nm
(3) Determination of the content of cholic acid in the sample: Take 10-15 mg of cholic acid sample, accurately weigh it, add a little 60% acetic acid to grind and dissolve it
.
If necessary, it can be heated and dissolved in a hot water bath, transferred to a 25mL measuring flask, diluted to the mark with 60% acetic acid solution, and shaken
8.
Product use
Pharmaceutical intermediates
.
Sodium cholate is a choleretic, which treats cholecystitis, bile deficiency, and intestinal dyspepsia
9.
Safety measures
(1) The bile used as the raw material for extracting cholic acid should not be stored for a long time to ensure the yield and product quality
.
Operators should wear labor protection equipment, and pay attention to ventilation and fire prevention in the operation place
(2) Store in a dry and airtight manner
Related links: Product performance and production process of cholic acid
