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Chinese scientists achieve direct measurement of the electrostatic interaction of proteins in cells

 Last Update: 2022-01-10
 Source: Internet
 Author: User

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Most proteins perform their functions in cells, and the complex cell environment can affect the electrostatic interactions of proteins, thereby affecting the performance of their functions, but the extent of the effects is unclear

The researchers used the cell core magnetic resonance technology and the double mutational cycle (double mutational cycle, DMC) method to study the 8 pairs of charge interactions in the intracellular IgG binding protein GB3, and the measurement results were compared with the static electricity measured in the buffer.

The results of this study reflect the influence of the intracellular environment on protein interactions, highlighting the importance of directly studying protein interactions and protein functions in cells

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