Cell Rep: Successfully cultured conjunctival tissue in a laboratory environment

February 3, 2021 / --- conjunctivation tissue is perhaps the most recognizable for the average person and is an important part of eye function and protection.
a new study, researchers at Osaka University produced functional conjunctivular tissue in-body by identifying key protein factors that drive the development and maturation of conjunctivular cells.
is a complex organ made up of several different types of tissues, each with a specific purpose to ensure the full function of the eye.
healthy tear production is an important factor affecting eye function and ensures that the eye does not dry.
dry eye disease is common in modern people and can cause inflammation and inflammation of the eyes, which can damage vision.
tear fluid is maintained by the epithe of the conjunctiva, a thin layer of cells that covers the inside of the sclea and eyelids.
to understand conjunctivism biology and find new treatments for dry eye disease requires the development of functional conjunctivic tissue.
the many eye diseases affect the parts of the eye that are directly related to the visual process, such as the crystal, retina and retina,.com" said Rhyuhei Hayashi, co-author of the study.
study, we focused on the tissue arranged outside the eyeball, which is just as important for proper vision.
our goal is to identify factors that are critical to the production of functional conjunctival tissue.
to achieve their goals, the researchers asked two questions: 1) how to form conjunctivular cells in the body; To answer these questions, the researchers used human-induced omnic stem cells (hiPSCs), which can be manipulated to make them any cell in the human body.
researchers from hipSCs produced cell self-forming outer embryo layer autonomy multi-region (SEAM), a cell structure that closely mimics the development of the whole eye in the disk.
researchers focused on specific cell substations in the SEAM that play the role of conjunctival cells.
then, they found that the development of conjunctivular cells required protein epithal growth factors (EGFs), while the maturation of conjunctivular tissue required protein cartics to form cell growth factors (KGFs).
, however, can the newly created conjunctivic tissue replicate its corresponding function in the living eye? To answer this question, the researchers investigated whether the tissue contained cup cells that produce mucous proteins, which play a vital role in the function of the human conjunctivitis.
they were able to show that their tissues, made up of hipsC and SEAM, contained a sufficient number of cup cells, suggesting that this was a good model for studying the biology and disease of human conjunctivums on plates.
" show how to closely simulate the structure and function of the eye in-body.
our findings will help to understand the biological properties of conjunctivitis and may further help model drug screening and regenerative therapies to combat the effects of dry eye disease.
" (Bioon.com) Source: Generation of conjunctivae in a dish Original source: "Generation of functional conjunctival epithelium, including goblet cells, from human ipsCs" Cell Reports (2021). DOI: 10.1016/j.celrep.2021.108715