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Understanding how proteins bend, twist, and deform in cells is very important for understanding normal biology and disease
"This is the first time a technology has been developed, and I think it may have a very big impact," said Dr.
Shoot very small things
This new method solves a basic problem that many molecules in living cells cannot be directly and accurately observed with ordinary optical microscopes
A technology called FRET can embed label pairs in the target protein, and their light will change as the protein conformation changes
The new Binder-tag method "tags" the inserted small molecule protein using a separate molecular binding tag (the tag contains the protein and needs a certain shape or structure), such as the protein when it actively helps the cell perform a specific function
The method is suitable for a wide range of tags, and Hahn says that Binder-tags can even be used to make FRET sensors more easily
According to Hahn, this is a powerful technique that, in principle, can handle a wide range of protein dynamics studies, including the study of proteins that are rarely found in cells
In a paper published in Cell, the research team discussed several proofs of principle
Hahn said: "Through this method, we can see, for example, how differences in the microenvironment between cells affect the activity of proteins, and often have far-reaching effects
Now, researchers are using this technology to draw dynamic maps of other important proteins
Scientists envision that Binder-tag will eventually become a basic technology for studying normal proteins, larger multi-molecular structures in cells, and even dysfunctional proteins related to Alzheimer's disease
"For many protein-related diseases, scientists still can't understand why proteins start to do the wrong thing
Original search:
Biosensors based on peptide exposure show single molecule conformations in live cells
DOI
10.