Cell: new tools are expected to accelerate antibody recognition and vaccine development and research, or to benefit multiple diseases such as HIV

January 6, 2020 / bioun / -- recently, in a research report published in the international journal Cell, scientists from Vanderbilt University have developed a new tool or are expected to accelerate the research of antibody vaccine through research and development; this tool, called Libra SEQ, may be expected to rapidly expand the field of antibody discovery and vaccine development research Photo source: cell, volume 179, issue 7, p1636-1646.e15, December 12, 2019 at present, due to technical limitations, researchers may spend one year to identify a single antibody from hundreds of millions of antibodies produced by B cells, and then develop it into a drug to identify and attack antigen markers on specific pathogens; therefore, researcher Ian Setliff and his colleagues wanted to know if they could map both antibody sequence and antigen specificity in a high-throughput way It sounds like a crazy idea and may not work, said researcher Georgiev, but now researchers have realized that in just a few weeks, they feel guilty and can find a new anti HIV antibody The development and validation of Libra SEQ tool may be able to link B cell receptor antigen specificity by sequencing The researchers say that it's usually difficult to know the antibody sequence, and we don't know the antigen specificity of the antibody In this paper, the researchers confirmed Libra SEQ technology through joint research using cell lines carrying B-cell receptor, and then the researchers are expected to carry out prospective antibody discovery research, that is, to determine the previously unknown broad neutralizing antibody spectrum that can resist HIV This new tool can speed up the discovery of antibodies, because researchers can use a larger screening library than other screening methods Researchers believe that Libra SEQ will be a revolutionary technology, which can have a more direct impact on basic research and application research, and can also put forward many questions that researchers could not answer before At present, researchers are studying new targets in the field of HIV infection and vaccine development They are very interested in revealing how antibodies recognize antigens The development of Libra SEQ technology may help them get a lot of data to solve these problems Libra SEQ technology can help researchers narrow down the range of targets that affect human health, which is of great significance for later scientists to develop new therapies against various human diseases Original source: Ian Setliff, Andrea R shiakolas, Kelsey a pillewski, et al High throughput m app ing of B cell receiver sequences to antigen specificity, cell (2019) Doi: 10.1016/j.cell.2019.11.003