Blood: Immunogenomic identification and characteristics of mony myeloma granulocytes inhibition cells

Granucytomyde-inhibiting cells (G-MDSCs) promote tumor growth and immunosuppression of multiple myeloma (MM)However, its pintype cannot be accurately detected and used for clinical transformationthis study aims to provide g-MDSCs phenotypes based on prognostic significance, immunosuppression potential, and molecular proceduresdetected pre-established G-MDSCs phenotypes in bone marrow samples from normal humans and MM patients through multi-dimensional flow cytokines, and surprisingly, the researchers found that CD11b-CD14-CD15-CD13-HLADR-cells overlapped with eosinophils and neutrophils commonly found in MM patientsAs a result, the researchers relied on automatic clustering to fairly identify all the granulocytic subgroups in the tumor microenvironment: alkaline granulocytes, eosinophils, immature neutrophils, neutrophils, and mature neutrophilsin 267 newly diagnosed MM patients (GEM2012MENOS65 trial), only the proportion of mature neutrophils at the time of diagnosis was significantly associated with the prognosis of the patient, and the high maturity of neutrophils/T cell ratio led to poor progression survival (P 0.001)of facing each neutrophil subgroup of FAC, t-cell proliferation decreased significantly in the presence of mature neutrophils (0.5 x, p.016), and the cytotoxicity of T-cells activated by BCMAxCD3 bispecific antibodies increased significantly with the depletion of mature neutrophils (4 times; P-0.0007)most interesting, RNAseq of the three subgroups showed that, due to differences in chromatin, G-MDSCs-related gene specificity in mature neutrophils in patients with multiple myeloma increased compared to the control group, the researchers established a clear correlation between the clinical significance, immunosuppressive potential, and transcription networks of neutrophil subgroups, providing for the first time a set of optimal markers (CD11b/CD13/CD16) that accurately monitors G-MDCS in MM