Bioclean safety cabinets serve as protective barriers for researchers, and these precautions must be known

　　Pharmaceutical Network Technical Article: Bioclean Safety Cabinet is a box-type air purification negative pressure safety device that can prevent the occurrence of aerosol dispersion in certain hazardous or unknown biological particles during experimental operation and processing It is widely used in microbiology, biomedical, genetic engineering, biological products and other fields of scientific research, teaching, clinical testing and production, is the laboratory biosecurity in the first level of protection barrier basic safety protection equipment
In the course of the fight against the new corona epidemic, the bioclean safety cabinet is the protective barrier of scientific researchers, which can protect the operating samples from outside pollution at the same time, better protect the operator from the operation of pathogenic microorganisms
Bioclean safety cabinet in the course of work is mainly the cabinet air pumping out, so that the cabinet to maintain a negative pressure state, through vertical air flow to protect the staff, external air through the efficient air filter filter into the safety cabinet to avoid the treatment of sample contamination, the cabinet air also needto be filtered through HEPA filter before discharge into the atmosphere, to protect the environment
Bioclean safety cabinets not only serve as a good protective barrier in this outbreak, but are also essential to today's microbial laboratories, especially when they require protective measures for operators Such as medical, pharmaceutical, scientific research and other bacterial culture to provide a sterile, dust-free and safe working environment However, when using biosafety cabinets, it is necessary to pay attention to the following things: 1 Do not block the front reception or the rear wall near the intake grille 2 Before removing the arm from the safety cabinet, be sure to carry out surface disinfection treatment, and then slowly move (moving direction perpendicular to the work area opening) 3 Clean items should be placed at least 150mm away from the aerosol sprig, reduce the possibility of cross-contamination 4 Minimize the movement of the arm Move slowly, prevent interference in the air flow inside the cabinet 5 The instruments that will produce air flow shocks, such as mixers, centrifuges or locators, placed close to the back wall of the cabinet 1/3 of the 6 Once spills occur during the experiment, use disinfection paper towels to be placed on its surface adsorption clean-up 7 Biowaste bags should be placed in the safety cabinet cannot take out the safety cabinet 8 The surface seal of the test tube or test plate containing the sample to prevent the sinking air flow from attacking the sample 9 Experimental operation as close as possible to the safety cabinet for bioclean safety cabinet widely used in medical and health care, disease prevention and control, food hygiene, biopharmaceuticals, environmental monitoring and various biological laboratories and other fields, can be divided into first, second and third levels, to meet different research and epidemic prevention requirements
Among them, the first-level biosafety cabinet can protect the staff and the environment, but not the sample The secondary biosafety cabinet is currently used for a wide range of cabinet types At present, the world's high security level safety cabinet for three levels, in fact, for the four-level laboratory biosecurity level designed, suitable for high-risk biological experiments
In addition, bio-clean safety cabinet as a widely used laboratory equipment, in use need to master three key points: 1, biosafety cabinet placement location The air flow rate from the opening of the front face of the biosafety cabinet into the cabinet is approximately 0.45 m/s As ideal, should be kept away from the movement and possibly interference with the air flow Try to leave 30 cm of space on the back and sides of the safety cabinet for easy maintenance work Above the cabinet is left with a height of 30 to 35 cm to measure the wind speed of the exhaust filter and leave enough room for the replacement of the exhaust filter
2, biosecurity cabinet decontamination treatment Decontamination of level 1 and 2 biosecurity cabinets is to place the appropriate amount of low polyformaldehyde (so that the concentration of low polyformaldehyde in the air in the cabinet is 80%) on an electric hot plate or frying pan (controlled from outside the cabinet) Another electric hot plate or frying pan with 10% more bicarbonate than polyformaldehyde is also placed in the cabinet (controlled outside the cabinet) This second container should be covered, which can be removed from a distance (e.g tied to a line that can be pulled from outside the cabinet) This allows low leveling of low formaldehyde in advance
3, biosafety cabinet cleaning and disinfection All items in the biosafety cabinet, including equipment, should be decontamination and removed from the cabinet after the work is complete, as residual culture media may provide microbial growth conditions The inner surface of the safety cabinet should be decontamination before and after each use To wipe the surface and inner wall of the workbench with disinfectant, the disinfectant used should kill all microorganisms that may be present in the cabinet At the end of the day, surface decontamination should be performed, i.e a thorough wipe of the workbench, on each side, back and inside the glass