ARD: In vitro mass spectrometry analysis reveals bone marrow pro-inflammatory features in the synovial fluid of psoriatic arthritis

Purpose : Many immune cell groups are involved in the pathogenesis of psoriatic arthritis (PsA) .
It is currently known that PsA is related to CD8+ T cells (especially those that produce IL-17 ); the role of myeloid cell population has not been explored to the same extent .
In this study, mass spectrometry flow cytometry (CyTOF) combined with transcriptomics analysis was used to generate matched high-dimensional data sets of PsA synovial fluid (SF) and blood leukocytes, with the purpose of identifying cells in vitro in unstimulated lymphoid and bone marrow cells Factor generation .

Purpose : Many immune cell groups are involved in the pathogenesis of psoriatic arthritis immunity (PsA) .

It is currently known that PsA is related to CD8+ T cells (especially those that produce IL-17 ); the role of myeloid cell population has not been explored to the same extent .
In this study, mass spectrometry flow cytometry (CyTOF) combined with transcriptomics analysis was used to generate matched high-dimensional data sets of PsA synovial fluid (SF) and blood leukocytes, with the purpose of identifying cells in vitro in unstimulated lymphoid and bone marrow cells Factor generation .

Method : Fix fresh SF and matched blood or incubate with protein transport inhibitor for 6 hours
.

The samples were stained with two CyTOF panels: a phenotypic panel and an intracellular panel, including antibodies against T cells and myeloid cells secreted proteins

Method : Fix fresh SF and matched blood or incubate with protein transport inhibitor for 6 hours

Results : The study observed that the myeloid compartment of PsA SF has undergone significant changes relative to blood, and the intermediate monocytes, macrophages and dendritic cell populations have expanded

Conclusion: Using multi-omics analysis, the graduate student has become a comprehensive cell atlas of PsA SF and blood to reveal the key bone marrow pro-inflammatory modules of PsA that are potentially pathogenic and therapeutically important

Source:

Yager N, Cole S, Lledo Lara A , et al .

Ex vivo mass cytometry analysis reveals a profound myeloid proinflammatory signature in psoriatic arthritis synovial fluid.
Annals of the Rheumatic Diseases2021; 80: 1559-1567.

Yager N, Cole S, Lledo Lara A , et al .
Ex vivo mass cytometry analysis reveals a profound myeloid proinflammatory signature in psoriatic arthritis synovial fluid.
Annals of the Rheumatic Diseases  2021; 80: 1559-1567.
, et al Annals of the Rheumatic Diseases  80: Leave a message here