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Application of bioreactors in the study of in vitro culture of bioengineered liver organs

 Last Update: 2022-09-14
 Source: Internet
 Author: User

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Functional characterization of a bioengineered liver after heterotopic implantation in pigs reports a method of re-endothelializing HUVECs (human umbilical vein endothelial cells) and implanting primary pig liver cells into a bioengineered liver (BEL) scaffold, using bioreactors to culture proliferative organ cells in vitro to obtain a bioengineered liver

First, pig livers are perfused separately using Triton X-100 and SDS detergent solutions to obtain cell-free scaffolds

Human umbilical vein endothelial cell culture with stent inoculation

Human umbilical vein endothelial cells are also pretreated (37 °C, 5% carbon dioxide) in antibiotic-free endothelial cell growth medium and then inoculated for culture

Fresh pig livers are enzymatically digested using collagenase and neutral protease after removal of residual blood and prophylaxis

Medium samples from the bioreactor are collected daily for offline analysis to determine the level of

Histological analysis, ammonia clearance kinetics analysis, and urea production assays confirmed that the bioengineered liver obtained by culture was capable of producing albumin, ammonia detoxification, and urea synthesis, with similar functions

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