Apoptosis of Hep2 cells induced by Cistanche deserticola polysaccharide extract
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Last Update: 2014-10-29
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Source: Internet
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Author: User
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Recently, researchers from the Department of Otorhinolaryngology, Affiliated Hospital of Yan'an University published a paper to study the antitumor effect of boschnica deserticola polysaccharide extract (BRP) on human laryngeal cancer Hep2 cells It was found that BRP inhibited the growth of Hep2 cells mainly through the change of cell cycle and apoptosis, and its pathway mainly included the internal pathway of mitochondria and the external pathway of death receptor This article was published in the 8th issue of Shaanxi medical journal in 2014 The components of polysaccharide extract were analyzed by high-throughput chromatography, cell cycle and apoptosis protein were analyzed by flow cytometry, and apoptosis related protein was detected by Western blot High throughput chromatography showed that BRP was a single component, and the inhibition rate of BRP was time and concentration dependent Cell cycle analysis showed that BRP kept cells in G0 / G1 phase Compared with the control group, different concentrations of BRP (100-400 μ g / ml) significantly induced apoptosis Western blot showed that after BRP, pro-caspase-3, pro-caspase-8 and pro-caspase-9 protein division increased, while the expression of death receptor DR5 and Bax increased, while Bcl-2 decreased.
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