Analysis of exosomal microRNA expression profile in urine of AMEM Alzheimer's disease 5XFAD mouse model

BACKGROUND Alzheimer's disease (Alzheimer's disease, AD) is a refractory and irreversible neurodegenerative disease, the pathogenesis of which is still unclear
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Therefore, before the deposition of amyloid-β plaques (Aβ), the identification of candidate biomarkers is of great significance for the early intervention of AD
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Researchers from the Pathology Platform Task Force of the Institute of Medical Experimental Animals of the Chinese Academy of Medical Sciences have determined the microRNA (miRNA) in the urine exosomes of the early Alzheimer mouse model to find markers that can identify AD in the early stage
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In this paper, a 1-month-old 5XFAD mouse model and its littermate control group in the early stage of Aβ plaque formation were selected to collect exosomes in urine and analyze the differential expression of miRNAs in them, using bioinformatics methods to analyze the differences The expressed genes were displayed, and at the same time, KEGG pathway analysis and GO enrichment analysis were performed on the differentially expressed genes screened out
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The results showed that: 48 differentially expressed miRNAs (18 up-regulated, 30 down-regulated), of which miR-196b-5p, miR-339-3p, miR-34a-5p, miR-376b-3p, miR-677-5p and miR -721 -6 miRNAs are predicted to show gene targets and important signal pathways closely related to the pathogenesis of AD
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The above conclusions were verified by ddPCR and a consistent conclusion was obtained
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 Figure 1 Workflow A of miRNA microarray analysis of urine exosomes in 5 XFAD Alzheimer's disease mouse models, sampling and isolation of urinary exosomes, and miRNA microarray evaluation
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B.
A block diagram showing the distribution of microarray data samples
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C.
Correlation graphs of extraurinary body samples in the 5XFAD model and the fat control group
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At the end of this article, combined with the molecular analysis of miRNA target interaction network, it is used to analyze the 5XFAD mouse AD model of urinary exosomes miRNA expression profiling
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Visualize the network of experimentally verified target genes and their miRNA functional annotations, and the node size reflects the degree of connection
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Figure 2 Through clustering, scatter plot, KEGG pathway analysis and regulatory network analysis, the expression of urinary exosomal miRNA is different in the 5XFAD mouse model before Aβ plaque deposition
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These exosomal miRNAs represent potential non-invasive biomarkers and can be used as reliable non-invasive sources of early AD biomarkers, and can be used in clinical applications to prevent the development of AD in the future
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Figure 3 Urine exosomes miRNA chip analysis of differentially expressed candidate miRNAs in the 5XFAD mouse AD model Med.
2021; https://doi.
org/10.
1002/ame2.
12175 [Health Sciences] About the journal "Animal Models and Experimental Medicine (English)" (Animal Models and Experimental Medicine, AMEM) by the Chinese Society of Experimental Animals, Chinese Medicine Co-sponsored by the Institute of Medical Laboratory Animals of the Academy of Sciences, and published in cooperation with the internationally renowned publishing group Wiley
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