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An innovative analysis solution for neuronal structure and activity: long-term + real-time quantification

 Last Update: 2022-08-30
 Source: Internet
 Author: User

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Why is dynamic assessment of neuronal activity important?

Analyzing a single endpoint (such as immunohistochemistry) during the entire process of neuronal maturation is not only impossible to perform repetitive kinetic assessments, but also requires tedious steps for fixing, staining, rinsing, collecting and analyzing images;
Nor can it be combined with the physiologically relevant environment to obtain comprehensive information;
Unable to confirm cellular morphology, spatial resolution, or assess functional connectivity;
Only a limited number of cells can be measured, and the real network environment cannot be simulated
.

Incucyte® provides innovative analysis solutions for long-term, real-time, continuous quantitative monitoring Incucyte® real-time live cell analysis system NeuroTrack software module

1 Using real-time phase contrast imaging to track the growth of different types of neuronal processes

2 Record the process of inducing the differentiation of human pluripotent stem cells into sensory neurons

Changes of iPSCs were observed by dynamic imaging 72h after adding Doxycycline

Assessment of Glutamate-Induced Neuronal Toxicity Using Incucyte® Cytotox Red

4 Characterization of neuronal activity and structure in neuron-glial co-culture system using neuronal labeling reagents NeuroLight and Annexin V

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- References - (1) Nickolls AR, Lee MM, Espinoza DF, Szczot M, Lam RM, Wang Q, Beers J, Zou J, Nguyen MQ, Solinski HJ, AlJanahi AA, Johnson KR, Ward ME, Chesler AT, Bönnemann CG.
Transcriptional Programming of Human Mechanosensory Neuron Subtypes from Pluripotent Stem Cells.
Cell Rep.
2020 Jan 21;30(3):932-946.
e7.
doi: 10.
1016/j.
celrep.
2019.
12.
062.
PMID: 31968264; PMCID: PMC7059559.

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