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Amplification of Genomic Sequences Flanking T-DNA Insertions by Thermal Asymmetric Interlaced Polymerase Chain Reaction

 Last Update: 2021-01-16
 Source: Internet
 Author: User

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define chain reaction

direct recovery

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Thermal asymmetric interlaced polymerase chain reaction (TAIL-
PCR
) is an efficient tool for the recovery of
DNA
fragments adjacent to known sequences. A protocol is presented for the amplification of genomic sequences flanking DNA (T-DNA or transposon) insertions using the TAIL-PCR method. The amplified products are suitable as templates for direct sequencing, or for cloning in vectors. Examples are given in use of the protocol for T-DNA tagging in rice using the pCAMBIA binary vectors.

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