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Agrobacterium-Mediated Transformation of Stem Disks from Oilseed Rape (Brassica napus L.)

 Last Update: 2020-11-20
 Source: Internet
 Author: User

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The commercial importance of oilseed rape (
Brassica nupus
L.) makes it desirable to have an efficient transformation protocol for practical applications. With the exception of a few reports, transformation experiments with
B. napus
using
Agrobacterium tumefaciens
yielding transgenic plants have been performed principally with the spring variety
B. napus
L. ssp. oleifera cv. Westar (
1
–
5
). Transformation protocols are relatively specific for cv. Westar and cannot be extended to most of the other spring and none of the winter varieties tested (
6
). There are only isolated reports on transgenic winter oilseed rape plants (
6
–
8
).

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