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Transcranial ultrasound stimulation (TUS) is a new generation of neuromodulation technology with great potential
.
Transcranial ultrasound can pass through the skull non-destructively and be focused on the millimeter-level deep brain region.
It has shown great potential in human brain function research and disease treatment
.
How to achieve precise ultrasound neuromodulation is an important research direction at present
.
Methods currently under development include: ultrasound acoustic genetics [1], ultrasound thermal genetics [2] and so on
.
This article provides a new way to go in parallel with it
.
"Gas vesicles (GVs)" is a unique gene-encoded gas-rich protein nanostructure, which is mainly expressed in aquatic photosynthetic organisms as a means of regulating buoyancy [3]
.
It has unique acoustic properties.
In recent years, a series of studies have shown its great potential in ultrasound molecular imaging, and it is considered to be the "fluorescent protein" in ultrasound imaging [4]
.
GVs can be targeted and expressed in specific locations or cells through minimally invasive delivery (microinjection or ultrasound blood-brain barrier opening) and gene editing technology under development
.
On September 21, 2021, Sun Lei's team from Hong Kong Polytechnic University published an article Precise Ultrasound Neuromodulation in a Deep Brain Region Using Nano Gas Vesicles as Actuators in Advanced Science, using low-frequency and low-intensity ultrasound to drive nano gas vesicles to achieve precision and reversibility.
And repeatable nerve regulation
.
GVs will vibrate in the sound field.
Researchers try to use this phenomenon to concentrate ultrasound energy near GVs, so that lower ultrasound intensity can be used to achieve targeted regulation of deep brain regions
.
The results of the study show that GVs may not only be the "fluorescent protein" in ultrasound, but also the "ChR2" in ultrasound other than the mechanically sensitive ion channel protein, which is expected to mediate ultrasound imaging and stimulation at the same time
.
Figure 1.
Schematic diagram of the experiment
.
GV vibrates under an ultrasonic field, triggering the opening of mechanically sensitive ion channels on the surface of neurons, and calcium ion influx, activating neurons in the deep brain area of mice
.
This study first started with isolated primary neuronal cells and used calcium imaging as a research method to prove that GV+US triggers calcium influx and neuronal activation
.
Adding a mechanosensitive ion channel blocker (RR) can reduce the calcium response, indicating that GV+US triggers the opening of the mechanosensitive ion channel and mediates the influx of calcium ions
.
In addition, the results of in vivo studies in mice show that GV+US can precisely regulate neurons in deep brain regions, displaying reversible and repeatable calcium responses, and the corresponding changes in neuronal calcium signals can be recorded by Fiber Photometry (FP).
The control group There is no similar reaction
.
The recorded calcium signal response is within 250 ms of the ultrasound transmission delay time, showing that the stimulation method has a high time resolution
.
The researchers tested the neuron activation marker c-Fos.
When GV was injected into the Ventral Tegmental Area (VTA) in the deep brain area of mice, the expression of c-Fos was mainly concentrated in the VTA area, while the control group showed little c-Fos Expression, which indicates that GV+US can successfully activate neurons in the deep brain area and has spatial targeting
.
Based on the above conclusions, the researchers demonstrated a biogenic nanobubble-mediated ultrasound precision neuromodulation technology, which lays a solid foundation for the basic research and clinical application of deep brain neuromodulation in the future
.
Professor Sun Lei from the Department of Biomedical Engineering of the Hong Kong Polytechnic University is the corresponding author of the paper.
PhD students Hou Xuandi, Dr.
Qiu Zhihai, and Dr.
Xian Quanxiang are the co-first authors of the paper
.
SUN Lab, Department of Biomedical Engineering, The Hong Kong Polytechnic University, is committed to the research of scientific issues in the intersection of biology, physics, and engineering.
It recruits postdoctoral fellows, doctoral students, and research assistants to develop methods and tools for ultrasound neuromodulation
.
The use of ultrasound to regulate nerve activity and nerve function is an emerging non-destructive brain stimulation and brain regulation method.
In the past two years, it has been considered as having great potential as photogenic technology
.
We will conduct in-depth and systematic exploration of the mechanism of nerve cell function and metabolism from the perspective of ultrasound, and develop precise ultrasound control and acoustic genetic methods and tools that have targeted specificity for specific neurons or neural circuits, so as to promote its development.
Therapeutic application of neurological and neurodegenerative diseases (such as Parkinson's disease, epilepsy, etc.
)
.
Researchers with a solid background in neurobehavioral, neurobiology, and cell and molecular biology are welcome
.
Preference is given to researchers who have optogenetic, neural circuits, and neurological and neurodegenerative diseases related research, have a strong interest in scientific research, and have solid English and writing skills
.
Resume delivery (if you are interested, please send your resume, transcripts and representative scientific research results to): https://jinshuju.
net/f/ZqXwZt or scan the QR code to deliver your resume.
Laboratory introduction: This laboratory is based on the non-destructive effect of ultrasound , Deep penetration, focusable, biological effects, etc.
, to carry out mechanism exploration, biomedical applications, system development and other research related to ultrasound neuromodulation and acoustic genetics
.
Based on ultrasound-based immune regulation, ultrasound/photoacoustic functions and molecular imaging are other major research directions in the laboratory
.
Professor Sun received a number of government research funding, including GRF, HMRF, ITF, NSFC, 973 and so on
.
Currently in Biomaterials, Advanced Science, Cell Reports, npj Regenerative Medicine, Acta Biomaterialia, iScience, Journal of Endocrinology, IEEE BME, Ultrasound in Medicine and Biology, IEEE UFFC, Medical Physics and other journals published articles
.
Original link: https://doi.
org/10.
1002/advs.
202101934 Plate maker: Eleven references [1].
Qiu, Z.
et al.
Targeted neurostimulation in mouse brains with non-invasive ultrasound.
Cell Reports 32, 108033 (2020).
[2].
Yang, Y.
et al.
Sonothermogenetics for noninvasive and cell-type specific deep brain neuromodulation.
Brain Stimulation 14, 790-800 (2021).
[3].
Shapiro, MG et al.
Biogenic gas nanostructures as ultrasonic molecular reporters.
Nature nanotechnology 9, 311-316 (2014).
[4].
Farhadi, A.
et al.
Ultrasound imaging of gene expression in mammalian cells.
Science 365, 1469-1475 (2019).
Notes for reprinting【 Non-original articles] The copyright of this article belongs to the author of the article.
Personal forwarding and sharing are welcome.
Reprinting is prohibited without permission.
The author has all legal rights and offenders must be investigated
.
.
Transcranial ultrasound can pass through the skull non-destructively and be focused on the millimeter-level deep brain region.
It has shown great potential in human brain function research and disease treatment
.
How to achieve precise ultrasound neuromodulation is an important research direction at present
.
Methods currently under development include: ultrasound acoustic genetics [1], ultrasound thermal genetics [2] and so on
.
This article provides a new way to go in parallel with it
.
"Gas vesicles (GVs)" is a unique gene-encoded gas-rich protein nanostructure, which is mainly expressed in aquatic photosynthetic organisms as a means of regulating buoyancy [3]
.
It has unique acoustic properties.
In recent years, a series of studies have shown its great potential in ultrasound molecular imaging, and it is considered to be the "fluorescent protein" in ultrasound imaging [4]
.
GVs can be targeted and expressed in specific locations or cells through minimally invasive delivery (microinjection or ultrasound blood-brain barrier opening) and gene editing technology under development
.
On September 21, 2021, Sun Lei's team from Hong Kong Polytechnic University published an article Precise Ultrasound Neuromodulation in a Deep Brain Region Using Nano Gas Vesicles as Actuators in Advanced Science, using low-frequency and low-intensity ultrasound to drive nano gas vesicles to achieve precision and reversibility.
And repeatable nerve regulation
.
GVs will vibrate in the sound field.
Researchers try to use this phenomenon to concentrate ultrasound energy near GVs, so that lower ultrasound intensity can be used to achieve targeted regulation of deep brain regions
.
The results of the study show that GVs may not only be the "fluorescent protein" in ultrasound, but also the "ChR2" in ultrasound other than the mechanically sensitive ion channel protein, which is expected to mediate ultrasound imaging and stimulation at the same time
.
Figure 1.
Schematic diagram of the experiment
.
GV vibrates under an ultrasonic field, triggering the opening of mechanically sensitive ion channels on the surface of neurons, and calcium ion influx, activating neurons in the deep brain area of mice
.
This study first started with isolated primary neuronal cells and used calcium imaging as a research method to prove that GV+US triggers calcium influx and neuronal activation
.
Adding a mechanosensitive ion channel blocker (RR) can reduce the calcium response, indicating that GV+US triggers the opening of the mechanosensitive ion channel and mediates the influx of calcium ions
.
In addition, the results of in vivo studies in mice show that GV+US can precisely regulate neurons in deep brain regions, displaying reversible and repeatable calcium responses, and the corresponding changes in neuronal calcium signals can be recorded by Fiber Photometry (FP).
The control group There is no similar reaction
.
The recorded calcium signal response is within 250 ms of the ultrasound transmission delay time, showing that the stimulation method has a high time resolution
.
The researchers tested the neuron activation marker c-Fos.
When GV was injected into the Ventral Tegmental Area (VTA) in the deep brain area of mice, the expression of c-Fos was mainly concentrated in the VTA area, while the control group showed little c-Fos Expression, which indicates that GV+US can successfully activate neurons in the deep brain area and has spatial targeting
.
Based on the above conclusions, the researchers demonstrated a biogenic nanobubble-mediated ultrasound precision neuromodulation technology, which lays a solid foundation for the basic research and clinical application of deep brain neuromodulation in the future
.
Professor Sun Lei from the Department of Biomedical Engineering of the Hong Kong Polytechnic University is the corresponding author of the paper.
PhD students Hou Xuandi, Dr.
Qiu Zhihai, and Dr.
Xian Quanxiang are the co-first authors of the paper
.
SUN Lab, Department of Biomedical Engineering, The Hong Kong Polytechnic University, is committed to the research of scientific issues in the intersection of biology, physics, and engineering.
It recruits postdoctoral fellows, doctoral students, and research assistants to develop methods and tools for ultrasound neuromodulation
.
The use of ultrasound to regulate nerve activity and nerve function is an emerging non-destructive brain stimulation and brain regulation method.
In the past two years, it has been considered as having great potential as photogenic technology
.
We will conduct in-depth and systematic exploration of the mechanism of nerve cell function and metabolism from the perspective of ultrasound, and develop precise ultrasound control and acoustic genetic methods and tools that have targeted specificity for specific neurons or neural circuits, so as to promote its development.
Therapeutic application of neurological and neurodegenerative diseases (such as Parkinson's disease, epilepsy, etc.
)
.
Researchers with a solid background in neurobehavioral, neurobiology, and cell and molecular biology are welcome
.
Preference is given to researchers who have optogenetic, neural circuits, and neurological and neurodegenerative diseases related research, have a strong interest in scientific research, and have solid English and writing skills
.
Resume delivery (if you are interested, please send your resume, transcripts and representative scientific research results to): https://jinshuju.
net/f/ZqXwZt or scan the QR code to deliver your resume.
Laboratory introduction: This laboratory is based on the non-destructive effect of ultrasound , Deep penetration, focusable, biological effects, etc.
, to carry out mechanism exploration, biomedical applications, system development and other research related to ultrasound neuromodulation and acoustic genetics
.
Based on ultrasound-based immune regulation, ultrasound/photoacoustic functions and molecular imaging are other major research directions in the laboratory
.
Professor Sun received a number of government research funding, including GRF, HMRF, ITF, NSFC, 973 and so on
.
Currently in Biomaterials, Advanced Science, Cell Reports, npj Regenerative Medicine, Acta Biomaterialia, iScience, Journal of Endocrinology, IEEE BME, Ultrasound in Medicine and Biology, IEEE UFFC, Medical Physics and other journals published articles
.
Original link: https://doi.
org/10.
1002/advs.
202101934 Plate maker: Eleven references [1].
Qiu, Z.
et al.
Targeted neurostimulation in mouse brains with non-invasive ultrasound.
Cell Reports 32, 108033 (2020).
[2].
Yang, Y.
et al.
Sonothermogenetics for noninvasive and cell-type specific deep brain neuromodulation.
Brain Stimulation 14, 790-800 (2021).
[3].
Shapiro, MG et al.
Biogenic gas nanostructures as ultrasonic molecular reporters.
Nature nanotechnology 9, 311-316 (2014).
[4].
Farhadi, A.
et al.
Ultrasound imaging of gene expression in mammalian cells.
Science 365, 1469-1475 (2019).
Notes for reprinting【 Non-original articles] The copyright of this article belongs to the author of the article.
Personal forwarding and sharing are welcome.
Reprinting is prohibited without permission.
The author has all legal rights and offenders must be investigated
.
