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Activation Tagging with En/Spm-I /dSpm Transposons in Arabidopsis

 Last Update: 2020-11-07
 Source: Internet
 Author: User

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stable

dna positive negative

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Activation tagging is a powerful strategy to find new gene functions, especially from genes that are redundant or show lethal knock-out phenotypes. It has been applied using T-
DNA
or transposons.
En
/
Spm-I
/
dSpm
engineered transposons are efficient Activation tags in
Arabidopsis
. An immobilized transposase source and an enhancer-bearing non-autonomous element are used in combination with positive and negative selectable markers to generate a population of single or low copy, stable insertions. This method describes the steps required to select the best parental lines, generate a population of stable insertions, and gene identification.

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