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Figure: Single-cell rotational adhesion frequency assay (scRAFA) measures adhesion kinetics of cells undergoing light-driven out-of-plane rotation near the substra.
Ligand-receptor binding is important for biological processes such as immunity and infectious diseas.
However, the differences in binding between P-selectin and ACE2 determined by different methods were significa.
Cell adhesion in living organisms is more complex than existing metho.
Preliminary studies suggest that integrins require lateral forces to mediate cell-to-cell interactio.
Flow cell assays, using existing techniques, cannot precisely control cell-substrate distanc.
Published in eLight , a team of scientists led by Associate Professor Yuebing Zheng from the University of Texas at Austin investigated the control of cell surface receptors (Figure
It enables label-free and subcellular resolution quantification of single cell adhesion of virtually any target in clinical solutio.
Researchers capture specific cells through seamless fusion of optical rotation and on a single platfo.
The light-driven scRAFA system integrates a range of functions, including optical trapping, rotation, imaging, and spectrosco.
Rotational adhesion and shear force measurements of cells with uniform and non-uniform surfac.
Most importantly, their scRAFAs can be applied to organisms under different physiological conditions (F.
The wider application of scRAFAs will require further modeling of organisms and their interactions with substrat.
