A high-throughput solid-phase extraction method for the detection of DON in wheat flour

DON, also known as deoxynivalenol (DON), is a trichothecenes compound.

Image source: Photo Network

Coma Bio uses the self-developed 24-well Copure® 230 multifunctional purification plate to achieve rapid and high-throughput detection of DON

An LC detection method for the detection of DON in wheat flour was established.

Refer to "GB 5009.

1.

1.

1) Weigh 2 g of sample, add 20 mL of acetonitrile-water solution (9+1) and mix well

2) After vortexing for 15 min, centrifuge at 6000 r/min for 5 min, and take the supernatant for purification

1.

Copure® 230 Multi-Function Cleaner (Cat.

1) Place the 24-well purification plate on the 24-well collection plate, and then add 6 mL of sample extract to the wells of the purification plate

2) Place the 24-well purification plate and the collection plate under the 24-well positive pressure extraction device, and turn on the air valve switch to keep the purification plate holes and the gas delivery holes corresponding and match well

3) Adjust the gas output pressure so that the sample extract is filtered into the collection plate

4) Take 4 mL of sample purification solution, blow dry with nitrogen at 40°C, reconstitute with 1 mL of initial mobile phase, vortex for 30s to dissolve the residue, pass through a 0.

Equipment: UltiMate 3000 with DAD detector

(Thermo Fisher Scientific)

Column: Agilent ZORBAX C18 

(4.

Mobile phase: A: methanol B: deionized water

Mobile phase gradient: see Table 1

Flow rate: 0.

Column temperature: 35℃

Injection volume: 50.
0 mL

Detection wavelength: 218 nm


Table 1 Mobile phase elution procedure

3.
Experimental results

Figure 1 Chromatograms before and after treatment with the Multi-Purpose Cleanup Plate

①Wheat Flour Sample-Uncleaned ②Copure® 230 Multi-Purpose Purification Plate-Cleaned 

It can be seen from Figure 1 that after the Copure® 230 multi-functional purification plate purification treatment, the impurities in the upper liquid are obviously adsorbed, there are fewer impurity peaks in the chromatogram, there are no interfering peaks next to the target peak, and the quantification is more accurate; The recovery rate of DON is between 90-110%, and the CV value of recovery rate between wells is less than 5%, which can meet the needs of experimental use
.

Table 2 The experimental results of standard addition and recovery by LC method

Figure 2 Chromatogram of spiked sample (200 ng/g) after cleanup using the Copure® 230 Multi-Purpose Cleanup Plate
 

4.
Ordering Information