10×Single-cell transcriptome common Q&A (1)--the most important questions to know before the experiment is carried out

There are many factors to consider during the early planning stages, but sample preparation is an important starting point

Figure 1 Single-cell experiment flow

Q2: Does single-cell sequencing require biological replicates?

Due to the influence of genetic and environmental factors, there will be differences between individuals, so the experimental design needs to introduce biological replication to eliminate this difference

Q3: How many cells are needed per sample?

At present, the single cell suspension needs to be checked and counted first.

Zhongke is equipped with 10×Genomics’ latest single-cell instrument platform, Chromium X, which has 16 channels, and each channel can capture up to 20,000 single cells

Q4: Why choose 10×Genomics for single-cell sequencing?

In the current single-cell market, why we choose 10×Genomic, the reasons are as follows:

A: The number of articles published by 10×Genomics single-cell technology is far ahead of scientists

B: More comprehensive applications to meet the needs of researchers in single-cell multi-omics

C: Higher throughput, lower cost

Figure 5 10×Genomics advantage map

Q5: What if the sample type is not suitable for transportation?

The single-cell experiment team of Zhongke can provide teachers with on-site digestion and computer services

Usually we have two ideas for cell type annotation, one is to use software to automatically annotate, such as SingleR, etc.
, but the results of automatic annotation can only be used as a reference; the other is to manually annotate based on literature or databases.
Professional manual annotation is usually regarded as is the gold standard for cell annotation, but the process is extremely time-consuming and slow, and can be subjective
.
Zhongke can provide the above two different annotation methods for teachers to choose!

Figure 7 Cooperative customer cell annotation diagram